PSII (Photosystem II)
PSI/PSII ratio can be estimated once a quantitative immunoblot for PsaC (AS10 939) and for either PsbA (AS05 084) or PsbD (AS06 146), is performed in 3-4 replicates. Ratio calculation is done on a sample by sample basis. These ratios are based on the subunits present, whether they are in the assembled photosystem or not. Under some circumstances (eg. high light), there are biological reasons that the subunit numbers do not necessarily match the functional photosystems.
Literature
Brown et al. (2008). Flux capacities and acclimation costs in Trichodesmium from the Gulf of Mexico. Marine Biology 154 (3): 413-422.
Levitan et al. (2010). Regulation of nitrogen metabolism in the marine diazotroph Trichodesmium IMS101 under varying temperatures and atmospheric CO2 concentrations. Environmental Microbiology 12(7): 1899-1912.
Thamatrakoln et al. (2013). A ‘Death-Specific Protein’ in a marine diatom regulates photosynthetic responses to iron and light availability. Proceedings of the National Academy of Science 110(50): 20123-20128 Li G,
Li et al. (2014). The Nitrogen Costs of Photosynthesis in a Diatom under Current and Future pCO2. New Phytologist, 205:533-543.
Protocols and Technical Information • Support • Western Blot Video Tutorials
AS08 300 | Extraction buffer for quantitative isolation of total soluble/membrane protein from plant tissue and algal/bacterial cells, optimized for subsequent western blot detection in denatured conditions
AS22 4733 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
AS06 111 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Cyanobacteria
AS22 4788 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
AS07 240 | Clonality: Polyclonal | Host: Rabbit | A.thaliana, H. vulgare, N. benthamiana
AS05 084A | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms compartment marker of thylakoid membrane
Benefits of using this antibody
AS01 016 | Clonality: Polyclonal | Host: Hen | Reactivity: [global antibody] for higher plants, algae, cyanobacteria and dinoflagellates
Benefits of using this antibody
AS05 084 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms | cellular [compartment marker] of thylakoid membrane
Benefits of using this antibody
AS05 084A-ALP | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms compartment marker of thylakoid membrane
Benefits of using this antibody
AS05 084B | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms
compartment marker of thylakoid membrane
Benefits of using this antibody
AS05 084A-FITC | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms compartment marker of thylakoid membrane
Benefits of using this antibody
AS05 084A-HRP | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms compartment marker of thylakoid membrane
Benefits of using this antibody
AS10 704 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, C.reinhardii, H.vulgare, M.truncatula, N. oceanica, N. oleoabundans UTEX 1185 (chlorophyta), P.patens, P.sativum, S.vulgaris, S.alba, S.oleracea, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803, Triticum sp.
AS11 1786 | Clonality: Polyclonal | Host: Rabbit | Reactivity: higher plants, alage, cyanobacteria
Benefits of using this antibody
AS13 2669 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants
Benefits of using this antibody
AS04 038 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, Physcomitrella patens, algae, cyanobacteria, diatoms
Benefits of using this antibody
AS11 1787 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, C.reinhardtii, C. zofingiensis, E. crus-galli, H. vulgare, O. sativa, P. ginseng, P. patens, P. sativum, P. vulgaris, P. yezoensis, Synochococcus sp. PCC7002, Synechocystis sp. PCC6803, T. aestivum, Triticale, Z. mays, V. lychnitis, V. radiate
AS06 146 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for A. thaliana, Anabaena 7120, D. brightwellii, H. vulgare, C.reinhardtii, C. zofingiensis, L. corniculatus, N. tabacum, O. sativa, P. sativum, P. vulgaris, P. tricornutum, T. pratense, S. alba, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803, T. guillardii, T. pseudonana, Triticale, U. prolifera, Z. mays
Benefits of using this antibody
AS06 112 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, H. vulgare, N.tabacum, O. sativa, S. oleracea | cellular [compartment marker] of thylakoid membrane
AS19 4330 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Synechocystis sp. PCC6803
AS06 113 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, P.sativum, T. aestivum
AS06 157 | Clonality: Polyclonal | Host: Rabbit | Reactivity: plants including A. balsamea, A.thaliana, H.vulgare, P.strobus, S.oleracea, Synechococcus sp. PCC 7942, Z.mays
AS10 692 | Clonality: Polyclonal | Host: Rabbit | Reactivity: cyanobacteria
AS06 158 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, H.vulgare
AS14 2786 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
AS05 092 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, N. tabacum, P. sativum, Z. mays
AS21 4689 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii, Synechococcus elongatus, Synechocystis sp.