It is crucial to know as much as possible about the target protein, before starting experimental work with detection using Western blot. Why is that? There are major differences in the protocol to apply, depending on the amount of target protein in the analyzed sample. For proteins of low expression, the following is recommended:
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Latest
Which isotype is a rabbit polyclonal antibody?2024-05-06 Why bands on the blot are not distinct from each other?
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